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iqtm syber green supermix  (Bio-Rad)


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    Structured Review

    Bio-Rad iqtm syber green supermix
    Iqtm Syber Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 17624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iqtm+syber+green+supermix/iQ+SYBR+Green+Supermix/pmc11926022-184-14-17
    Average 98 stars, based on 17624 article reviews
    iqtm syber green supermix - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: Oxidative stress response and programmed cell death guided by NAC013 modulate pithiness in radish taproots
    Article Snippet: For each RNA sample, a 20 μl mixture of 1 μg total RNA, 1 μl oligo(dT)16 (10 p m μl −1 ), 1 μl dNTP (10 m m ), 4 μl 5 × first‐strand buffer, 1 μl dithiothreitol (100 m m ), 1 μl SuperScript III reverse transcriptase (200 U μl −1 ; Invitrogen) and RNase‐free water was prepared. .. The resulting cDNA library was diluted by 25‐fold and mixed with iQTM SYBER Green Supermix (Bio‐Rad, Hercules, CA, USA) for qRT‐PCR experiments on a BioRad CFX96 Real‐Time PCR detection system. .. The resulting cDNA library was diluted by 25‐fold and mixed with iQTM SYBER Green Supermix (Bio‐Rad, Hercules, CA, USA) for qRT‐PCR experiments on a BioRad CFX96 Real‐Time PCR detection system.

    Real-time Polymerase Chain Reaction:

    Article Title: Oxidative stress response and programmed cell death guided by NAC013 modulate pithiness in radish taproots
    Article Snippet: For each RNA sample, a 20 μl mixture of 1 μg total RNA, 1 μl oligo(dT)16 (10 p m μl −1 ), 1 μl dNTP (10 m m ), 4 μl 5 × first‐strand buffer, 1 μl dithiothreitol (100 m m ), 1 μl SuperScript III reverse transcriptase (200 U μl −1 ; Invitrogen) and RNase‐free water was prepared. .. The resulting cDNA library was diluted by 25‐fold and mixed with iQTM SYBER Green Supermix (Bio‐Rad, Hercules, CA, USA) for qRT‐PCR experiments on a BioRad CFX96 Real‐Time PCR detection system. .. The resulting cDNA library was diluted by 25‐fold and mixed with iQTM SYBER Green Supermix (Bio‐Rad, Hercules, CA, USA) for qRT‐PCR experiments on a BioRad CFX96 Real‐Time PCR detection system.

    Article Title: Prevalence of Trypanosoma and Sodalis in wild populations of tsetse flies and their impact on sterile insect technique programmes for tsetse eradication
    Article Snippet: .. The amplification mixture contained 5 μl of DNA template, 200 nM of each primer, and 7.5 μl iQTM SYBER Green Supermix (Bio-Rad). qPCR cycling conditions for Sodalis were as follows: initial denaturation at 95 °C for 2 min; 39 cycles of 95 °C for 5 s, 55 °C for 30 s, one step at 95 °C for 5 s and a melting curve constructed from 65 °C to 95 °C in increments of 0.5 °C for 5 s. The same conditions were used for Trypanosoma except the annealing temperature was at 60 °C. ..

    Article Title: Influences of thermal stress on the growth biometrics, stress indicators, oxidative stress biomarkers, and histopathological alterations in European seabass, Dicentrarchus labrax , juveniles
    Article Snippet: The reverse transcriptions were carried out using an iScriptTM cDNA synthesis Kit (Bio-Rad, Hercules, CA, USA) to produce cDNA in a 20 μL reaction using 1 μL of total mRNA at a concentration of 0.5 μg/μL. .. In a final volume of a 20 μL reaction, which contained 10 μL of iQTM-SYBER® Green Supermix (Bio-Rad, Hercules, CA, USA), 5 μL of free-nuclease water, 3 μL of cDNA (1:10 dilution), and 1 μL of forward and reverse primer, the real-time PCR analysis was carried out using an iCycler with the optical module. ..

    Article Title: Prophage-dependent recombination drives genome structural variation and phenotypic heterogeneity in Escherichia coli O157:H7
    Article Snippet: 200 ng of DNase treated RNA was then converted to cDNA using iScriptTM Reverse Transcription Supermix (Bio-Rad) according to manufacturer guidelines. .. All qPCR reactions were carried out using iQTM Syber® Green supermix (Bio-Rad) and stx2a specific primers (IDT-DNA): stx2a-F–GAAGAAGATGTTTATGGCGGTTT, stx2a-R–CCCGTCAACCTTCACTGTAA. ..

    Article Title: Influences of thermal stress on the growth biometrics, stress indicators, oxidative stress biomarkers, and histopathological alterations in European seabass, Dicentrarchus labrax, juveniles.
    Article Snippet: The reverse transcriptions were carried out using an iScriptTM cDNA synthesis Kit (Bio-Rad, Hercules, CA, USA) to produce cDNA in a 20 μL reaction using 1 μL of total mRNA at a concentration of 0.5 μg/μL. .. In a final volume of a 20 μL reaction, which contained 10 μL of iQTM-SYBER® Green Supermix (Bio-Rad, Hercules, CA, USA), 5 μL of free-nuclease water, 3 μL of cDNA (1:10 dilution), and 1 μL of forward and reverse primer, the real-time PCR analysis was carried out using an iCycler with the optical module. ..

    other:

    Article Title: Method for evaluating efficacy of chemoradiotherapy against squamous cell carcinoma
    Article Snippet: The diluted cDNA was mixed with iQTM SYBER (registered trademark) Green Supermix (BIO-RAD Laboratories, Inc.), primers, and nuclease-freewater, and quantified using MyiQ (registered trademark) (BIO-RAD Laboratories, Inc.).

    Amplification:

    Article Title: Prevalence of Trypanosoma and Sodalis in wild populations of tsetse flies and their impact on sterile insect technique programmes for tsetse eradication
    Article Snippet: .. The amplification mixture contained 5 μl of DNA template, 200 nM of each primer, and 7.5 μl iQTM SYBER Green Supermix (Bio-Rad). qPCR cycling conditions for Sodalis were as follows: initial denaturation at 95 °C for 2 min; 39 cycles of 95 °C for 5 s, 55 °C for 30 s, one step at 95 °C for 5 s and a melting curve constructed from 65 °C to 95 °C in increments of 0.5 °C for 5 s. The same conditions were used for Trypanosoma except the annealing temperature was at 60 °C. ..

    Construct:

    Article Title: Prevalence of Trypanosoma and Sodalis in wild populations of tsetse flies and their impact on sterile insect technique programmes for tsetse eradication
    Article Snippet: .. The amplification mixture contained 5 μl of DNA template, 200 nM of each primer, and 7.5 μl iQTM SYBER Green Supermix (Bio-Rad). qPCR cycling conditions for Sodalis were as follows: initial denaturation at 95 °C for 2 min; 39 cycles of 95 °C for 5 s, 55 °C for 30 s, one step at 95 °C for 5 s and a melting curve constructed from 65 °C to 95 °C in increments of 0.5 °C for 5 s. The same conditions were used for Trypanosoma except the annealing temperature was at 60 °C. ..

    Isolation:

    Article Title: Donor Mesenchymal Stem Cells Program Bone Marrow, Altering Macrophages, and Suppressing Endometriosis in Mice
    Article Snippet: .. Total RNA was isolated from BM or endometriotic lesions using TRIzolTM reagent followed by purification using RNeasy® spin columns. qRT-PCR was performed with the cDNA using iQTM SYBER® Green Supermix (Bio-Rad) with specific primers for each gene. ..

    Purification:

    Article Title: Donor Mesenchymal Stem Cells Program Bone Marrow, Altering Macrophages, and Suppressing Endometriosis in Mice
    Article Snippet: .. Total RNA was isolated from BM or endometriotic lesions using TRIzolTM reagent followed by purification using RNeasy® spin columns. qRT-PCR was performed with the cDNA using iQTM SYBER® Green Supermix (Bio-Rad) with specific primers for each gene. ..

    Quantitative RT-PCR:

    Article Title: Donor Mesenchymal Stem Cells Program Bone Marrow, Altering Macrophages, and Suppressing Endometriosis in Mice
    Article Snippet: .. Total RNA was isolated from BM or endometriotic lesions using TRIzolTM reagent followed by purification using RNeasy® spin columns. qRT-PCR was performed with the cDNA using iQTM SYBER® Green Supermix (Bio-Rad) with specific primers for each gene. ..



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